The effect of Anti-Mullerian hormone on yield of oocytes recovered by ovum pick-up (opu) in heifers

The aim of this study was to determine the relationship between the oocyte yield by the ovum pick-up (OPU) technique with the Anti-mullerian Hormone (AMH) concentrations of the donors. Ten healthy Holstein heifers aged 12 to 15 months were included in the study. AMH measurements were performed with Bovine VIDAS® Anti-Mülleian Hormone kits (Biomeriux, Marcy l'Etoile, France) using the Mini Vidas device. A total of 67 OPU sessions were performed on a random day of the cycle. Oocytes were classified according to their quality, and viability evaluation of oocytes was made according to the cell layer number and cumulus integrity in the cumulus-oocyte complex (COC), the homogeneity of their cytoplasm. The average oocyte yield in OPU sessions per animal was range from 4–8. There was an significant negative correlation between the collected oocyte numbers and plasma AMH levels. In conclusion, it was observed that AMH concentration did not affect the number of viable and the quality oocytes collected in weekly OPU administration in animals. It was thought that OPU applications performed without knowing the day of the cycle did not provide the expected correlation with AMH data.