High Performance Liquid Chromatographic Technique and Validation for Determination of Favipiravir in Bulk and Tablet Formulation

Favipiravir is an antiviral that is active against many RNA viruses, and also used in COVID 19. A simple, economical, accurate, and precise HPLC method with UV detection was developed to quantify Favipiravir. It was resolved on the C18 column using the mobile phase blend of methanol acetonitrile ortho phosphoric acid in an isocratic mode, flow rate of 1.0 mL min with a proportion of 40 30 30 ,v v v. The detector wavelength was set at 322 nm. Reverse phase HPLC method, using UV detector is developed for the estimation of Favipiravir API. Used Liquid chromatographic system from Shimadzu comprising of Auto sampler, quaternary gradient pump for constant flow and constant pressure delivery and UV Visible detector connected to software Lab solutions for controlling the instrumentation as well as processing the generated data. A mixture of Methanol Acetonitrile Ortho phosphoric acid in the ratio 40 30 30 v v v was used in RP HPLC as diluent and as a mobile phase. Ms. Pritee R. Atkar | Dr. Mirza Shaihed Baig "High Performance Liquid Chromatographic Technique and Validation for Determination of Favipiravir in Bulk & Tablet Formulation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-7 , December 2022, URL: https://www.ijtsrd.com/papers/ijtsrd52424.pdf Paper URL: https://www.ijtsrd.com/pharmacy/other/52424/high-performance-liquid-chromatographic-technique-and-validation-for-determination-of-favipiravir-in-bulk-and-tablet-formulation/ms-pritee-r-atkar