DETERMINATION OF ANTIOXIDANTS FROM FOENICULUM VULGARE AND CUMINUM CYMINUM SEEDS

In this work we aimed to determine the antioxidant composition of the seeds and bulb with edible leaves of fennel (Foeniculum vulgare) and cumin seeds (Cuminum cyminum) using different methods. Hydroalcoholic extracts have been prepared from both seeds and the leafy stem of fennel, respectively from cumin seeds. From these extracts, the polyphenol content was determined using the Folin Ciocalteu quantitative method. The determination of the composition in antioxidants was made by reverse phase liquid chromatography with mobile phase B-Acetonitrile acidified with Formic Acid and A-Acidified water with Formic Acid, at pH 3. The detectors used were a mass spectrometer and a UV spectrophotometer. The antioxidant capacity was determined by the CUPRAC method, in which the reducing activity of antioxidants on Cu(II) ions at Cu(I) revealed by the formation of a complex with an adequate ligand was also determined spectrophotometrically.