ANTI-HEPATITIS C ACTIVITY OF EXTRACTED FRACTION OF PSEUDOMONAS OLEOVORANS USING HEPATITIS C VIRUS CELL CULTURE SYSTEM

One of the primary goals of antiviral research in recent decades has been the biosynthesis of new antiviral agents. The large number of bioactive agents extracted from microbial isolates led to the building of connections between products through antiviral screening. Using a genotype 4 plasmid, a microbial antiviral extract fraction from Pseudomonas oleovorans evaluated against HCV in Hepatitis C cell culture. The cytotoxicity assay using MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] for determination of the ability of the mitochondrial enzyme succinate dehydrogenase to breakdown the tetrazolium salt and reflect cell viability indicates the inhibitory concentration of 50% of the fraction is732.2 µg/mlto be further applied for the concentration conventionalin the antiviral assay. The antiviral assay screenedthrough inhibition of viral entry, interference with viral replication, and blocking viral assembly and release assays. In both viral cell pre-treatment and viral replication interference assays, the extract inhibited viral infection in HCV cell culture systems with reduction in extracted HCV viral RNA from 9,837 to <12 and 4,035 to < 12 IU/ml respectively. On the other hand, there was a slight reduction in viral RNA in case of inhibiting assembly and releasemechanism from 4950 to 3710 IU/ml after detecting RNA using Real-time PCR.