Impact of RAGE Gene rs80096349(C>T), rs1035798(C>T), and rs184003(G>T) Polymorphisms on Non-Response to Aspirin in Iraqi Patients with Coronary Artery Disease

Background: Transmembrane multiligand receptor RAGE is a protein found on the surface of cells that binds to the advanced glycation end products. Platelet cyclooxygenase activity is elevated in response to RAGE activation, contributing to the formation of a thrombus. Objectives: To examine the connection between RAGE gene rs80096349(C>T), rs1035798(C>T), and rs184003(G>T) polymorphisms and resistance to aspirin in CAD patients from Iraq. Patients and methods: Patients with coronary artery disease (CAD) (161 males and 64 females) were enrolled in the trial between February 2021 and October 2021. All participants were taking prophylactic Aspirin (100 mg). The control group consisted of 130 individuals, including 97 males and 33 females, who appeared to be in good health and were not taking aspirin. Serum thromboxane B2 levels were used to assess the effectiveness of aspirin (TBX2). Patients were classified as either aspirin sensitive or resistant. The polymorphism of RAGE's most closely related single nucleotide polymorphisms (SNPs) was identified using polymerase chain reaction for amplification of the extracted deoxyribonucleic acid and Sanger's method of sequencing. Results: A total of 225 cardiovascular patients compatible with inclusion criteria were enrolled. There were 161(71.6%) male and 64(28.4%) female patients with a mean age of 56.85±8.11years old. The statistical analysis revealed 17.8% (n = 40) aspirin-resistant cases in the study population. Genotypic analysis of our data showed that participants with the T allele of rs184003(G/T) had an increased prevalence in the aspirin-resistant group compared with the aspirin-sensitive group (p< 0.05). In contrast, for rs10835798, the frequency was dramatically higher for T alleles in aspirin sensitive group contrasted with the resistant group (p< 0.05). The aspirin-resistant group was found to have significantly greater RAGE levels than the sensitive group. Conclusion: Our results provide evidence that RAGE expression and gene rs1035798(C>T), and rs184003(G>T) polymorphisms could be predictors for CAD patients' resistance to aspirin.