Molecular Detection of bla TEM and bla CTX-M Genes in Clinical and Food-Borne Klebsiella pneumoniae Isolates

To isolate Klebsiella pneumoniae, two hundred clinical and food samples were cultivated on differential and selective culture media. 120 bacterial isolates were isolated from the food and clinical samples. On MacConkey agar, 81 isolates of lactose fermenters (67.5%) and 39 isolates of non-lactose fermenters (32.5%) were found. Out of 120 bacterial isolates discovered in clinical and food sources, 32 were K. pneumoniae isolates, including 14 from food sources and 18 from clinical sources. K. pneumoniae was determined to be the species of bacteria isolated based on biochemical testing, morphological characteristics, and cultural identities. This conclusion was confirmed by the Vitek system. The findings of this investigation, which used phenotypic methods, revealed a high frequency of isolates of K. pneumoniae that produce Extended Spectrum B-Lactamase (ESBL) from patients in Iraq and a low frequency of isolates from food sources. After bacterial DNA was isolated, pure DNA samples with concentrations between 76 and 91 ng/ml were produced. Utilizing particular primers for the genes bla TEM and bla CTX-M, this work carried out the molecular detection of two ESBL-coding genes. According to the findings of this study, bla TEM was only found in clinical isolates at a detection rate of 27.77% and bla CTX-M at 38.88%. Isolates from food sources tested negative for bla TEM and bla CTX-M.