Green Synthesis, Characterization and Anti-Helmintic (Worms) Potency of Copper (II) Oxide and Zinc (II) Oxide Nanoparticles Derived From Azadirachta Indica (Neem) Bark

Zinc (II) and copper (II) oxide nanoparticles (ZnO-Nps and CuO-Nps) were synthesized from Azadirachta indica (Neem) bark. The complexes were characterized, and their anti-helminths (worms) activities were studied. Direct synthesis was used for the synthesis and characterization using FTIR. UV-visible, XRD and SEM. In-vitro and In-vivo were used to test the biological activities (anti-helminthic). The UV-Visible absorption spectrum of zinc oxide nanoparticles shows the characteristic surface plasma resonance with a wavelength of approximately 200 – 440 nm and a peak maximum at 380 nm with attributes to the formation of nanoparticles. FT-IR Spectroscopy was used to identify the possible biomolecules responsible for reducing zinc nitrate ions and copper sulfate and capping the reduction of zinc nanoparticles. Copper nanoparticles synthesis used Azadirachta indica (Neem) bark extract and the result shows zinc oxide nanoparticles gave the IR bands 3220.4 cm-1, 2102.2 cm-1, 1990.4 cm-1, 1595.3 cm-1, 1401.5 cm-1, 1088.4 cm-1, 771.8 cm-1, 849.8 cm-1, 1028.7 cm-1,. The results observed from copper oxide nanoparticles are 3252.61 cm-1, 2918.62cm-1, 2850.90 cm-1, 2168.92 cm-1, 1636.30 cm-1, 1239.42 cm-1, 1083.80 cm-1, and 1035.91 cm-1. The bands which appeared at 3252.61cm-1 and 2918.62cm-1 corresponds to O-H stretching and symmetric C.H. stretching respectively. XRD shows sharp peaks of the nanoparticles, which indicates the formation of nanoparticles and crystallinity. SEM analysis also showed a magnified image of the nanoparticles, further confirming their formation. The nanoparticles were found to be potent in anti-helminthic activities at 200mg/ kg.