Design and Optimization of Everolimus Drug Loaded Protein Nanoparticles to Treat Glioblastomas

The experimental design is used to optimize the trials for Everolimus drug loaded with protein (Fibrinogen) nanoparticles in the design space in a way that produces reliable and consistent results with the fewest possible experiments in order to optimize the batches, the study was carried out to know the effects of independent variables by calculating their respective responses based on the trial runs performed. The design points are positioned at the middle of the subareas of the dimension k-1. The optimization studies were performed on 17 trial batches and the responses i.e., particle size and percent drug entrapment were evaluated. Out of the 17 batches it was observed that F14 batch depicted optimum results for the respective responses i.e., particle size and percent drug entrapment. The results of ANOVA along with response surface and contour plots were generated for response. Optimum results of particle size were observed to be 149.8±1.02 nm and 92.2±0.22 % drug entrapment.