ANTI-BACTERIAL, CYTOTOXICITY, AND ANTIOXIDANT PROPERTIES OF THE ISOLATED FLAVONOIDS EXTRACT FROM WHITE DRAGON FRUIT (HYLOCEREUS UNDATUS) PEELS AND FLESH

The presence of flavonoids in white pitaya's flesh and peel was confirmed using the Thin Layer Chromatography Screening method. Flavonoid was extracted using solvent fractionation and then subjected to the three assays. Using the Kirby-Bauer method, better antibacterial activity against E. coli was found using 100% peel extract, partially active at 10.28 mm. The flesh flavonoid extract was inactive against both E. coli and S. aureus at concentrations of 100%, 75%, and 50%. The flavonoid peel and flesh have the potential as anticancer agents since they are active, toxic, and potent, with LC50 of less than 1000 ppm in Brine Shrimp Lethality Assay. Using a UV VIS spectrophotometer at 517 nm, both the flesh flavonoid extract and the peel flavonoid extract have antioxidant potential. However, the flesh with a percent Radical Scavenging Activity close to the catechin control is a better antioxidant. This study proved that the peel and flesh of White Pitaya, particularly its flavonoid, have medical benefits, can be a potential source of income for farmers, and decrease waste in the environment.