Reduction in Methane Productions from Sheep by Supplemented of Moringa oleifera Leaf Extract in The Ration

This study aimed to evaluate the decreasing methane gas production in sheep by supplementing Moringa oleiferaextractin the ration. The study was conducted in a Completely Randomized Design with One Way Classification. The treatment of Moringa oleiferaextract was offered at 0, 0.25 and 0.50% of DMration, each with six replicates, which constitutes 18 treatment units. The materials included ruminal fluid of three freshly slaughtered thin-tailed sheep derived from an abattoir in Banyumas Central Java. Moringa oleiferaleaves were oven-dried at 60ºC for2 x 24 h,grounded into powder and extracted using ethanol. The ration consisted of concentrate and ammoniated rice straw with a ratio of 60:40 (DM basis). The concentrate was composed of 2:1 rice bran and cocconut meal. The measured variables includedtotal protozoa, methane gas production, total bacteria, N-NH3concentration, and protein synthesis of ruminal fluid. The result indicated 0.50% Moringa oleiferaextract (DMbasis) produced the lowest total protozoa, the highest total bacteria and protein microbe synthesis, and the lowest methane gas production