Analysis of Pork DNA in Non-Halal Certified Jamu Capsule Shells Using Polymerase Chain Reaction (PCR) Method

Jamu is an original Indonesian medicine, one of which is formulated in the form of capsules. The rise of products that are not certified halal is a problem that needs to be studied more deeply, especially regarding the halalness of a product. Capsules are produced from animal gelatin, the alleged use of pork gelatin is the core of the problem in this study. The purpose of this study was to determine the presence or absence of pork DNA in the shells of jamu capsules. The sample was taken through one of the e-commerce with certain criteria. From several products, 5 jamu capsules were selected, which were marked with samples A, B, C, D, and E. The five samples were isolated to obtain pure DNA which was then amplified using the right primer to form millions of DNA using the PCR method, and electrophoresis to see the DNA bands produced on agarose media and the number of base pairs (bp) which were then compared with positive controls. From the research conducted, it was found that there were 4 samples B, C, D, and E detected positive for containing pork DNA at 132 bp. Meanwhile, the jamu capsule shell preparation in sample A was negative for pork DNA.