Genosensor Study for Fetal Cell-Free DNA Detection |Biomedgrid

β-Thalassemia is one of the most monogenic autosomal recessive disorder characterized by defective production of the hemoglobins β-chain. Definition of the β-globin genotype is necessary for genetic counselling in the carriers, and for predicting prognosis and management options in the patients with thalassemia. Genetic analysis of β-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. The aim of this study is to develop a new procedure, a nanopolymer-based Lab-on-a-chip based biosensor for the real-time multiplex analysis of β thalassemia mutations from Cell-Free DNA. In this study, biospecific interaction analysis (BIA) employing quartz-cristal microbalance (QCM) and biosensor technologies was applied to the analysis of multiple mutations of the human β-globin gene. To this aim, large target polymerase chain reaction products were immobilized on genosensor electrode and then probes detecting IVSI-110 β-thalassemia mutations were sequentially injected.