Banking Progenitor Cells for Hippiatric Regenerative Medicine: Optimized Establishment of Safe and Consistent Cell Sources for Standardized Veterinary Therapeutic Protocols |Biomedgrid
Journal: American Journal of Biomedical Science & Research (Vol.8, No. 4)Publication Date: 2020-04-08
Authors : Alexis Laurent; Salim E Darwiche; Nathalie Hirt-Burri; Corinne Scaletta; Murielle Michetti; Philippe Laurent; Wassim Raffoul; Anthony S de Buys Roessingh; Lee Ann Applegate;
Page : 252-271
Keywords : Cell banking; cell therapy; hippiatric regenerative medicine; horses; musculoskeletal injuries;
Abstract
Acute musculoskeletal injuries in large animals such as horses or camels often result in productivity losses and euthanasia in racing, esthetic or stock animal industries. Bioengineered products for tissue reconstruction and wound healing may supplement traditional veterinary surgical care synergistically. Banked primary fetal progenitor cells can potentially be used towards structural and functional therapeutic restoration in condensed timeframes. Extensive clinical experience exists in our University Hospital working with dermal progenitor fibroblasts for managing burns, donor site grafts and ulcers. By extrapolation, the present study assessed suitability of equine fetal progenitor cell sources for biotechnological processing, robust cell banking and application in tissue engineering strategies for hippiatric regenerative medicine. Diverse fetal equine musculoskeletal tissues were obtained and processed under defined frameworks and protocols for standardized and optimized tiered cell bank establishment and characterization. Consistency, safety and cyto-compatibility of progeny cells with therapeutic delivery systems were assessed.
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