In vitro Evaluation of Osteoblasts Exposed to Different Bioceramic Root Canal Sealers

Background: This study aimed to evaluate the cytotoxicity and cell viability of two calcium silicate-based sealers. Material and Methods: Test specimens were made in a silicone matrix and divided into 4 groups (n=3) according to the sealer evaluated: control; Bio-C Sealer; EndoSequence BC and AH Plus. The sealers were weighed, sterilized with ethylene oxide, and cultured in Dulbecco's modified Eagle's medium supplemented with 15% fetal bovine serum and 1% antibiotic- antimycotic for 24 h (37±1°C). MTT and alkaline phosphatase (ALP) assays were performed in osteoblast-like SAOS-2 cells (Alizarin red staining). Data were analyzed by ANOVA and Tukey's test (p<0,05). Results: The greater cell vitality was observed in the control group at 24 h and in AH Plus at 48 h. At 72 h, there was no significant difference in MTT assay results between the groups (p>0.05). At 7 days, the control group had the best ALP assay result. At 10 days, the best results were found for the control group, Bio-C Sealer, and AH Plus, with a significant difference from EndoSequence BC (p<0.05). Conclusion: AH Plus sealer had higher levels of cell viability and bioactivity similar to the calcium silicate-based sealers, EndoSequence BC and Bio-C Sealer at 24 and 48 h.