DETERMINATION OF THROMBIN AND PLASMIN ACTIVITY IN HUMAN BLOOD PLASMA USING THE TURBIDIMETRIC CURVE OF CLOT FORMATION AND DISSOLUTION
Journal: Biotechnologia Acta (Vol.16, No. 2)Publication Date: 2023-04-28
Authors : A.V. UDOVENKO;
Page : 50-52
Keywords : thrombin; plasmin; blood plasma coagulation; hemostasis; coagulation; fibrinolysis.;
Abstract
The aim of the study was to develop a method for determination the activity of thrombin, which is based on the turbidimetry curve of the formation and dissolution of a blood plasma clot. Methods. Donor blood samples were collected in 3.8% sodium citrate (1 part of sodium citrate and 9 parts of blood, pH 7.4). Plasma was separated from blood cells within 1 hour after blood collection by centrifugation the latter at 1200 g for 20 minutes. Aliquots of plasma were stored at -20 °C. Results. To determine the concentrations of thrombin and plasmin, TDCs of the formation and dissolution of blood plasma clots, initiated by the APTT reagent, were used. Based on the values of τ obtained, a calibration curve was constructed in the coordinates 1/τ – [Thr] (the rate of protofibrils formation in s-1 vs thrombin concentration in NIH units in 1 ml). Conclusion. The proposed methods to determine the activity of thrombin and plasmin made it possible to quantitatively calculate the rate of prothrombin activation in the lag period, the concentration and activity of thrombin based on the rate of fibrin and protofibrils formation as well as the activity and concentration of plasmin at the point of the complete clot dissolution
Other Latest Articles
- FIBRINOLYTIC POTENTIAL INCREASING DURING ACTIVATION OF BLOOD COAGULATION IN THE COURSE OF PREGNANCY WITH PLACENTAL DYSFUNCTION
- STARVATION DURING DEVELOPMENT AFFECTS METABOLISM IN DROSOPHILA
- ROLE OF NO IN SOFT PERIODONTAL TISSUES OF RATS DURING STRESS AND INFLAMMATION
- Ex vivo STUDY OF THE ACTION OF INTEGRIN RECEPTORS ANTAGONIST FROM ECHIS MULTISQUAMATIS SNAKE VENOM ON PLATELETS OF PREGNANT WOMEN WITH COMPICATIONS DURING GESTATION
- THE SEARCH OF STRUCTURAL PATTERNS OF IVERMECTIN ALLOSTERIC INTERACTION WITH GLUTAMATE-GATED CHLORIDE CHANNEL OF Caenorhabditis elegans
Last modified: 2023-07-11 01:21:17