THE DNA THREAT PROBING OF SOME CHROMOPHORES USING UV/VIS SPECTROSCOPY

Chromosphere such as triaryl methane family of dyes used in various industries can affect the body of living organism system upon the interaction with the most biochemical molecule is the DNA. In this research, the binding mode of triaryl methane family of chromophore dyes that is Bromothymol blue, Bromophenol blue, Bromocresol green and Malachite green with double strand DNA in phosphate buffer at pH 6.9 was investigated through UV/VIS spectroscopy. The study shows that Bromothymol blue undergo non covalent interaction with DNA, bromocresol green interact through groove binding and malachite green and bromophenol blue interact through intercalative and partially through electrostatic mode of interaction with DNA. The studies on the binding nature of chemicals/dyes, which are regarded as small molecules to DNA are important and fundamental issues in life sciences. These dyes were found to be more toxic and care should be taken during disposing them off. Proper disposal of these dyes is needed for safe environment and healthy life.