Facile Synthesis of Copper Sulphide Nanoparticles using Trichoderma Harzianum and Antibacterial Efficiency Enhancement by Gentamicin Sulfate

The application of biosynthesized semiconductor nanomaterials has gained recent attention towards pathogenic bacteria. This study reports cost- effective, eco- friendly biosynthesis of copper sulphide nanoparticles (CuS NPs) employing the fungal cell mass of the biocontrol pest, T. harzianum. Later, gentamicin was loaded onto the mycosynthesised CuS NPs to assess the enhancement in its antibacterial activity. A synergistic antibacterial efficacy was observed for the GS- laden CuS NPs against the pathogens tested (E. coli, Salmonella Typhi, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus subtilis). The green synthesized CuS NPs as well as GS- laden CuS NPs were found to be relatively non- toxic towards MDA MB- 231 epithelial cell line. In short, the GS- laden CuS NPs were found to be an effective candidate to combat pathogenic microorganisms. The present study highlights the facile synthesis of semiconductor nanomaterials which could act as potential drug carrier and opens the feasibility of biocompatible materials for drug loading in the field of nanomedicine.