Study of Anti-Thrombocyte Activity of Cassia fistula Seeds Extract and It’s Total Phenolic and Flavonoid Content, In Vitro Antioxidant and Anti-Inflammatory Activities

The purpose of this study was to measure the total phenol and flavonoid content and assess the antioxidant and anti-thrombocyte activity in ethanol extracts of Cassia fistula seeds. Aluminum chloride was used to calculate the amount of flavonoids, and Folin-Ciocalteu reagent was used to calculate the total amount of phenolic compounds using a spectrophotometric method. The in vitro antioxidant activity of the analyzed extracts was evaluated utilizing the DPPH approach. The total phenolics and flavonoid contents in the seeds extract Cassia fistula 246 ± 0.08 mg GAE/g dw and 118 ± 0.001 mg QE/g dw, respectively. The obtained results concluded that it may be considered a good amount of phenolic and flavonoid compounds. The in vitro anti-inflammatory properties showed the highest percentage inhibition of protein denaturation was 54% for 45 µg/ml and 70% for the reference drug diclofenac sodium at a similar dose. The minimum inhibition of hemolysis 58% was observed at 50 µg/mL of Cassia fistula seeds extract and 81% for the same dose of standard aspirin. The results presented that the ethanol seeds extract of Cassia fistula has potential anti-thrombocyte activity. IC50 values were used to express the antioxidant activity of the investigated extracts. The IC50 values was found to be 8.90 µg/ml for ethanol seeds extract which is comparable to that of ascorbic acid (IC50 = 6.73 µg/ml) a well-known standard antioxidant. Among these results, the lower the IC50 showed the higher the free radical scavenging activity. Among these findings, the stronger the free radical scavenging activity was seen the lower the IC50 indicated. Based on all of the findings, we concluded that the anti-thrombocyte antioxidant and anti-inflammatory activities may be caused by phytochemicals identified in C. fistula extract.