The Ethanolic Extract of Basic Leaves (Ocimum basilicum) Effect on Expression of Follicle Stimulating Hormone Receptors (FSHr) and Folliculogenesis in Female Mice Model Endometriosis

Background: Endometriosis is a tissue disease similar to the endometrial lining, either glands or stroma that grows outside the uterus which is one of the causes of infertility due to a decrease in the amount of folliculogenesis. Purpose/Aims. This study aims to analyze the effect of administration of ethanol extract of basil leaves (Ocimum basilicum) on the expression of follicle stimulating hormone receptor (FSHr) endometriosis tissue and folliculogenesis in female mice model endometriosis. Method: Experimental research was conducted in the labomiceory with a simple experimental design (Posttest Only Control Group Design) involving two groups of subjects, one subject was treated (experimental group) with ethanol extract of basil leaves with a graded dose of 0.21mg/g-BW; 0.42 mg/g-BW; and 0.84 mg/g-BW and the other group was given a placebo (control group) by grouping 7 samples in simple random order per group. In the control group, 7 mices were terminated on day 15 to prove the endometriosis mice model. FSHr expression was evaluated from peritoneal tissue using immunohistochemistry and folliculogenesis was evaluated from ovarium using haematoxylin-eosin staining. Results: This study showed a decrease in the percentage of FSHr expression between the control and treatment groups (p=0.259), an increase in the percentage of the number of antral follicles (p=0.203) and the number of corpus-luteum with significant values ​​that were not significantly different (p=372) in endometriosis female mice model. Conclusion: There was a decrease in FSHr expression especially at the dose of 0.42 mg/g-BW and an increase in the number of antral follicles and corpus luteum especially at the dose of 0.21 mg/g-BW, but not significantly.