Inducible Nitric Oxide Synthase (iNOS) Appears Sensitive to In vitro Glucose Deprivation |Biomedgrid

Aging and cardiovascular disease (CVD) continue to be an intense area of research. Glycemic flux has long been reported to be linked with clinical outcomes associated with cardiovascular disease with much attention being given to type 2 diabetes (T2D) and elevated blood glucose levels. While it is established in models of glucose deprivation that CVD is an end-product, less is known about the molecular signaling events that lead to them. In this report, we subject C2C12 cells to durations of glucose deprivation and assess gene and protein expression the inducible nitric oxide synthase (iNOS) and upstream mitogen-activated protein kinase (MAPK) signaling proteins. Our data shows that iNOS appears sensitive to low glucose, but its upregulation may occur via a different pathway than the MAPK signaling cascade.