Assessment of Oxytetracycline Residue in Table Eggs from Khartoum State’s Markets |Biomedgrid
Journal: American Journal of Biomedical Science & Research (Vol.14, No. 1)Publication Date: 2021-08-25
Authors : Sara A M; Hala A E; Sahar M N; Samia A A Hassan; Inaam EL A Dawd; Hadeel A AMabyie; Samia H Abdulrahman; Iahraga G;
Page : 1-5
Keywords : HPLC; Oxytetracycline; Egg; MRL; Quantitation;
Abstract
Background: Antibiotics residue in food from animal origin represents a public health issue concerns the authorities worldwide.
Method: 100 egg samples were collected from Khartoum state's markets to evaluate the current situation of oxy tetracycline residue. briefly, Samples were homogenized, weighed (2 g); and processed by adding citric acid (100 mg), 30% nitric acid (1 ml), methanol (4 ml) followed by adding 1 ml of deionized water; then centrifuged at 5300 for 10 mints followed by filtration throughout 0.22 μm syringe filter and 20 μl of the sample was injected into HPLC equipped with UV-VIS detector and ODS (C-18) column and read at 360 nm.
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