ISOLATION AND CHARACTERIZATION OF PATHOGENIC PSEUDOMONAS SPECIES FROM URINARY TRACT INFECTION (UTI)AND WOUNDS IN SAUDI ARABIA AT MAKKAH REGION

Background: Pseudomonas aeruginosa(p.aeruginosa) is an opportunistic infection that causes severe urinary tract infection, it is well established That its common species in wound infection (chronic wound) is aeruginosa, here we studied the clinical isolates in urine and wound and their sensitivity pattern. Materials and Methods: This was a cross-sectional study aimed to isolate and characterizing the p. aeruginosa from urine and wound samples, 106 participants were enrolled for this study, patients under antibiotic and unconsented were excluded, bacteria were isolated with automated instrument, data was analyzed by SPSS V26, p.value<0.050 was considered significant data was expressed as percentage. Result: One hundred sex participants were enrolled for this study, n=77(72.64%) Saudi and n=29(27.35) was non-Saudi nationality. The male was n=56(52.83%), while female n=50(47.16%) study participants. We included the wound sample which comprises 49.05% and urine samples 50.94% table (1). Of 106 isolates of P. aeruginosa enrolled for this study, the overall drug resistance was moderate ranging from 6(5.66%) to 27(25.47%) to the all 13psudomonal antibiotic tested. Piperaquine is the lowest resistance in comparison to other antibiotics (5.66% p.value<0.050 while the ipeneme is the most resistant in comparison to the other 12 antibiotics 25.47% p.value<0.050. finally, the overall multiple drug resistance of P. aeruginosa was 16.11%. Conclusions: The clinical isolates of P. aeruginosa in urine and wound samples were moderate resistance further hospitals survey was required.