Alcohol and Advanced Glycation End Product Synergistically Stimulate TNF-ɑ and TGF-β1 Production by Cytochrome P450 2E1 Expressing Macrophages |Biomedgrid

Introduction: To study whether overexpression of CYP2E1 within macrophages is more sensitive to stimulators alcohol and AGEs by evaluation of TNF-ɑ and TGF-β1 generation. Methods: Transfected CYP2E1 cDNA (E2) and WT (M1) macrophages were cultured with alcohol and AGE. TNF-ɑ and TGF-β in culture medium was quantified using ELISA. Expressions of CD14, TLR2, TLR4, TLR9, and RAGE, and HO-1level were analyzed by Western blot. Cells were treated for 0-24 h for H2O2 and HO-1 analysis. siRNAs CD14, TLR4 and TLR9, and scrambled oligonucleotides were used to investigate role of different receptor-mediated oxidative stress in TNF-ɑ and TGF-β generation in E2 and M1 cells. Results: TNF-ɑ and TGF-β protein in E2 was significantly induced in cultures treated with alcohol or/and AGE compared to M1 or control cultures. Increased expression of CD14, TLR4, TLR9 and RAGE, and increased formation of H2O2 but decreased HO-1 level in CYP2E1-expressing macrophages were found when the cells were treated with alcohol and AGE. TNF-ɑ and TGF-β protein in E2 cells treated with siRNAs of RAGE, CD14, TLR4 and TLR9 were downregulated in the E2 and M1 cells.