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EFFECT OF OREGONIN ON THE MICROBIOME AND FORMATION OF THE AMINO ACID FUND OF THE INTESTINE, LIVER AND PLASMA

Journal: Journal of the Grodno State Medical University (Vol.21, No. 5)

Publication Date:

Authors : ;

Page : 477-482

Keywords : oregonin; amino acids; microbiocenosis; intestine; liver; plasma;

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Abstract

Background. This article analyzes the effect of a course intragastric administration of oregonin on the microbiocenosis of parietal mucin, the morphological structure of the intestinal mucosa, as well as the formation of the amino acid fund of the microbial-tissue complex of the small intestine, liver and plasma. Objective. The aim of the study was to identify the mechanisms of the biological activity of oregonin when administered to normal animals. Material and methods. The experiments were performed on white female rats weighing 180-240 g. The animals were enterally administered with 0.005% aqueous solution of oregonin at a dose of 5 mg/kg of body weight for 10 days daily. The control group received equivalent volumes of saline. In samples of the microbial-tissue complex of the small intestine, liver and plasma, the concentrations of free amino acids and their nitrogen-containing metabolites were determined by HPLC. Morphological and microbiological studies were carried out according to standard methods. Results. In animals treated with oregonin, there were no negative changes in the structure of the jejunal wall. In the microbiome of the parietal mucin, an increase in the population of anaerobes was observed, due to the growth of bifidobacteria and lactobacilli. At the same time, a bacteriostatic effect was recorded against lactose-negative and gas -forming microflora. In the microbial-tissue complex of the small intestine, after the enteral administration of oregonin, the total levels of amino acids and nitrogen-containing derivatives, which are produced mainly by facultative anaerobic microflora, decreased. In the liver, the intake of oregonin led to an increase in the total amount of amino acids and their derivatives. The total content of proteinogenic amino acids and their nitrogen-containing derivatives also decreased in the blood plasma. Conclusion. Oregonin demonstrates a high biological activity when administered enterally to animals. Positive changes in the intestinal microbiome and increased production of mucin, on the one hand, probably inhibit the production of nitrogen-containing products by facultative anaerobes, and on the other hand, initiate the use of free amino acids for biosynthetic purposes in tissues, while preventing their catabolism.

Last modified: 2023-12-18 16:52:06