EFFECT OF HYPOXIC SECRETOME MESENCHYMAL STEM CELLS ON TGF-β EXPRESSION, ESTROGEN HORMONE LEVELS, AND NUMBER OF FOLLICLES (IN-VIVO EXPERIMENTAL STUDY ON FEMALE WISTAR RATS POLYCYSTIC OVARY SYNDROME-LIKE MODELS)

PCOS develops when the ovaries are stimulated to produce more androgen hormones so that the development of follicle count is disrupted. Elevated insulin levels contribute directly to abnormalities in the pituitary hypothalamus of the ovaries which have a direct impact on the occurrence of PCOS by hyperinsulinemia mechanism that increases the frequency of GnRH (Gonadotropin Releasing Hormone) secretion, LH (Luteinizing Hormone) production exceeds FSH, increased ovarian androgen production, decreased follicle count maturation, and increased TGF-β (transforming growth factor β) expression. This study aimed to determine the effect of SH-MSCs administration on TGF-β expression, estrogen hormone levels, and follicle count in female Wistar strain rats with PCOS-like. This study is an in vivo experimental study using a post-test-only control group design. The subjects of this study were female Wistar strain rats divided into 4 treatments consisting of 1 group of healthy mice, 2 negative control groups, 3 treatment groups 1, and treatment group 2 with each different dose. Then the data was analyzed using the One Way Annova Test to determine the influence of each group.One Way ANOVA test showed the results of TGF-β expression (p = 0.000), then the results of estrogen hormone levels (p = 0.000), then the Kruskall Wallis test showed the number of cystic follicles (p = 0.045) in all groups there was a significant difference (p < 0.05) except cystic follicle.There is an effect of SH-MSCs secretome administration on decreasing TGF-β expression and caustic follicle count. There is an effect of SH-MSC administration on increasing estrogen hormone levels and corpus luteum count.