Standardization Studies of Momordica charantia Seed with Physicochemical and Preliminary Phytochemical Accounts

Momordica charantia Linn also known as Karela or bitter guard is commonly used vegetable of Cucurbitaceae family. Phytochemical constituent present in seeds contains oil, and sterols possesses the anti bacterial anti microbial and anti inflammatory properties which makes it one of the oldest plant used is natural Indian medicinal system. Present study was aimed to study organoleptic characterization, determination of moisture, total ash, acid-insoluble, water-soluble ash and protein contents, Loss on drying, heavy metals and microbial profile of Momordica charantia seed without seed coat. Furthermore qualitative test for secondary metabolites were performed for bitters, saponine, terpenoids, phenolic acids, glycosides, carbohydrates and reducing sugars were marked in preliminary tests. In final standardization extracts were taken for UV spectroscopy (at 270nm wavelength) at different concentrations against standard for quantitative analysis. After preparing different compositions mobile phase containing ethylene acetate: water: formic acid (7.7:1.3:0.9) was selected for TLC of chlorogenic acid marking in aqueous extract of Momordica charantia seed and gallic acid was run using mobile phase using toluene: formic acid: methanol (3.3: 0.8: 0.2) showed the presence of markers against standard. The plates were prepared and developed in mobile phase and Rf value was calculated. Photography of plates was done under UV visible mode. The above findings can be helpful in further research to reveal the reverse pharmacology of Momordica charantia seeds reported in traditional uses with significant way to design newer outcomes in diseases related with inflammatory and immune markers.