Biochemical study of freshwater fish Clarias batrachus (L.) infected with cestode parasite, Lytocestus sp. from district Jalgaon, India

This study involved analyzing bio-molecules including protein, glycogen and lipids in parasites as well as in both the infected and non-infected intestine of the host. The worms were sourced from the intestinal region of the freshwater fish Clarias batrachus and underwent a washing and other necessary processes. The dry weight was recorded after subjecting the samples to a temperature range of 50-60°C for duration of 24 hours. During the comparison between cestode parasites and the host intestine, it was observed that Lytocestus sp. displayed reduced concentration of protein and glycogen in comparison to both the infected and non-infected segments of the host's intestine. Moreover, the lipid concentration in the parasite exceeded that found in the host intestine, regardless of infection status. Author noticed that concentration of protein, glycogen, and lipids were notably high in the non-infected region compared to the infected section within the host intestine.