Effects of Turbulent Mixing and Orbitally Shaking on Cell Growth and Biomass Production in Active Fluids |Biomedgrid
Journal: American Journal of Biomedical Science & Research (Vol.15, No. 4)Publication Date: 2022-02-14
Authors : Zahra Samadi; Malihe Mehdizadeh Allaf; Reza Saifi; Christopher T De Groot; Hassan Peerhossaini;
Page : 396-404
Keywords : Photobioreactor (PBR); Photosynthetic microorganisms; Incubator; Incubator shaker; Magnetic stirrer;
Abstract
We have previously reported the effects of mixing on bacterial growth. Mixing can be achieved with different methods and in diverse devices. However, the nature of the intimate contact (the main purpose of mixing) made between different substances in a mixing vessel is a strong function of the hydrodynamic that underlies the specific mixing method. This study aimed to compare the growth and biomass yield of cyanobacterium Synechosystis sp. CPCC 534 (as a microorganism model) obtained by three different mixing methods commonly used in life science labs and in the industry, i.e., turbulent stirring (TS), orbital shaking (OS), and simple molecular diffusion (MD). The results revealed that imposing mixing on the culture significantly improved the specific growth rate as well as biomass yield production in comparison with simple molecular diffusion. Mixing obtained by turbulent stirring proved to be more efficient than the one achieved by orbitally shaking, in the production of Chlorophylla (Chla) and phycocyanin (PC). The results of this study can help choosing the appropriate mixing method in life science research.
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