Development And Validation of An In-House Brucella Smooth Lipopolysaccharide as A Coating Antigen in ELISA For Serodiagnosis of Bovine Brucellosis |Biomedgrid

Background: Brucellosis is an important zoonotic bacterial disease of global health importance affecting different animals and man. Brucellosis control and eradication procedures are highly depending on accurate diagnostic tools and effective and safe vaccination programs. Indirect ELISA, complement fixation tests, Buffered Acidified Plate Agglutination and the Rose Bengal plate agglutination are usually used for testing animals against bovine brucellosis. The aim of the work is to evaluate and validate the diagnostic efficacy of in-house prepared S-LPS as an IELISA coating antigen in comparison to commercial ELISA kit used for diagnosis of bovine brucellosis. Materials and methods: an Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) was developed using in house produced and titrated Smooth Lipopolysaccharide (S-LPS) as coating antigen and compared with the commercial kit using 115 negative and positive bovine sera. The performance parameters for each test were estimated using Complement Fixation Test (CFT) as a gold standard test.