Restoration of hepatorenal dysfunction and injury by zinc and folic acid combination in bisphenol A-intoxicated mice

Bisphenol A (BPA) is a potentially hazardous substance and is extensively used in manufacturing industries as a plasticizing agent. The current research intended to determine the revival actions of zinc (Zn) and folic acid (FA) on hematological parameters and hepatorenal function in BPA-exposed male albino mice. A total of 75 adult male mice, aged 25 to 28 days, were split into 5 groups. Group A (control group) received a normal diet, while a daily dosage of 50 mg BPA/kg body weight (BW) in the diet was provided to groups B–E. Groups C, D, and E received daily supplementation of 10 mg Zn/kg BW, 3 mg FA/kg BW, or both in the feed. After 12 weeks of treatment, serum was prepared by collecting blood, and the kidney and liver were taken for a histotexture study. The results demonstrated that hematological values dropped significantly (p< 0.05) in the BPA-treated group and increased following Zn and FA supplementations, while no significant alterations in erythrocyte indices were observed among control and treated groups. The BPA group used to have significantly (p< 0.05) higher liver and kidney biomarkers levels, which were restored by Zn and FA. The effects of BPA administration included severe hepatocyte degeneration and necrosis, as well as renal tubule epithelium loss, all of which were improved by Zn and FA. Hence, the findings suggest that combination of Zn and FA could be potential preventative agents against BPA-induced toxicity.