The Complex Infertility Treatment: Optimum Time for Oocyte Vitrification as Indicated by Meiotic Spindle, with Thawed Oocytes Fertilized by Intracytoplasmic Sperm Injection |Biomedgrid

Here we report thepregnancy of 38 years old women with low ovarian reserve whose spouse originally had no living sperms. The IVF/ICSI cycle was a first-choice treatment. In that case, collected oocytes had to be vitrified and thawed before fertilization. Oocyte polar body and optically birefringent meiotic spindle were visualized by polarized light microscopy and their states and relative positions were used as an indicators of full oocyte maturation, i.e., readiness for vitrification. According to our opinion this strategy enabled to vitrify fully mature (MII phase) oocytes, which were after thawing prepared for ICSI. Thawed oocytes were finally fertilized by intracytoplasmic sperm injection (ICSI) [1-3]. The 42 years old man spermiogram was improved by Tamoxifen treatment.