Assembly Formation of Synthetic DNA (E. coli) Crown Cells with Salt “Reconstruction and Regeneration of Synthetic DNA Crown Cells” |Biomedgrid

DNA crown cells (artificial cells) have an outer membrane covered with DNA and can be synthesized readily using sphingosine (Sph)-DNA-adenosine mixtures (synthetic DNA crown cells) and can proliferate within egg whites (DNA crown cells). Numerous synthetic DNA crown cells and DNA crown cells have been prepared using DNA from a variety of donor organisms. Although methods for preparing synthetic DNA crown cells and DNA crown cells have been established, how synthetic DNA crown cells proliferate within egg white remains unclear. This study clarified the mechanisms underlying the proliferation of synthetic DNA crown cells in egg white using salt and inorganic substances in egg white. The findings showed that the assemblies of synthetic DNA (E. coli) crown cells formed spontaneously and in the presence of salt and that round objects, or modified DNA crown cells, were also produced. These findings showed that synthetic DNA crown cells were reconstructed, formed assemblies, and regenerated synthetic DNA crown cells from the assembly.