Characterization and In Vitro Anti-cholesterol Activity Test of Ethanol Extract of Fruit Ficus racemosa L

The fruit of Ficus racemosa L., known in Indonesia as Loa, is believed to contain compounds that have biological activity. One of them is to lower cholesterol. This research aims to obtain initial information regarding the fruit ethanol extract's character, including secondary metabolite content, predicted compounds contained, water and ash content, and anti-cholesterol IC50. The methods used in predicting chemical compounds are using specific reagents and Gas Chromatography-Mass Spectrometry (GC-MS) instruments, water content using the Karl Fischer titration method and ash content using the gravimetric method, IC50 using the Visible spectrophotometric method. The test gives positive results containing alkaloid compounds, flavonoids, terpenoids, saponins, tannins, and glycosides with water and ash contents of 10.64% and 10.40%, respectively, and anti-cholesterol activity in vitro with an IC50 value of 522 µg/mL..