PROTEIN ANALYSIS FROM DIFFERENT BODY TISSUES OF THREE IMPORTANT COMMERCIAL RACES OF MULBERRY SILKWORM BOMBYXMORI(L)BY USING BRADFORD METHOD
Journal: International Journal of Advanced Research (Vol.12, No. 03)Publication Date: 2024-03-20
Authors : Ravikiran K.M Simi Simon Roopa K.B; Asiya Nuzhat F.B;
Page : 367-375
Keywords : Bombyxmori L. Protein Haemolymph Midgut Silkgland Fatbody;
Abstract
The mulberry silkworm Bombyxmori L. is one of the economically beneficial insect. Natural silk is produced by silkworm which converts mulberry leaf protein in to silk. Three mulberry silkworm races viz.,two single hybrid FC1(CSR6×CSR26), FC2(CSR2×CSR27)and one bivoltinedouble hybrid FC1× FC2[(CSR6×CSR26) × (CSR2×CSR27)] were selected for the present study. The main aim of present study was made an attempt to analyse and compare the protein content in Haemolymph, Midgut, Silkgland and Fatbodyselected silkworm races of fifth instar larvae. Data obtained were statistically analysed with two way ANOVA method at (p<0.001), (p<0.005) and (p<0.05) level of significance. High level of significance (p<0.001)were recorded in silkgland compared to haemolymph, midgut and fatbody in all selected silkworm races whichcomparing the protein content in haemolymph and midgut of all selected silkworm FC1× FC2races shows (p<0.001) significance compared to FC1 (p<0.005) and FC2 (p<0.05), protein content in silkgland and fat body shows (p<0.001) in FC1× FC2 compared to FC1 and FC2respectively. The results therefore, showed bivoltinedouble hybrid silkworm is superior in protein content in all examined different tissues and the study would be of great help to farmers to choose the better silkworm races for rearing purpose.
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