Genistein Preserves Pancreatic β-Cells Against H2 O2Induced Apoptosis in Mice Insulinoma Cell Lines |Biomedgrid

The plant-based isoflavones, particularly genistein, have been shown as a potent stimulator of insulin secretion with promising hypoglycemic activity; however, very little is known about its anti-apoptotic potential. The current pre-clinical investigations relate genistein as a β-cell protective agent, using in vitro model of H2O2-induced apoptosis in mouse insulinoma (MIN6) cells. Apoptotic induction was examined by annexin-V/propidium iodide staining, cleaved casp3 through immunostaining followed by fluorescent microscopy, whereas expressions of caspase 3 and caspase 9 genes by RT-PCR. We found that in genistein pre-treated conditions, few cells fall in the early and late stage of apoptosis as compared to H2O2-treatment only. In subsequent GS-treated MIN6 cells, almost no detectable levels of cleaved casp-3, an oxidative stress-mediated activator, confirmed the anti-apoptotic effect mediated by genistein. We observed a significantly lower level of casp-9; however, no effect on casp-3 expression in cells pre-treated with genistein. Moreover, genistein exhibited no cytotoxic effect at an effective concentration. The pre-clinical data revealed that genistein preserves pancreatic β-cells against H2O2-induced apoptosis in MIN6 cells and appeared suitable for further investigations.