IN VITRO CALLUS INDUCTION AND REGENERATION OF ORYZA SATIVA L. VAR. MTU1075 (PUSHYAMI)

In vitro inducement of callus and plant regeneration capacity were investigated using mature embryos of the Indian rice (Oryza sativa L.) variety MTU1075. The investigation was conducted using callus induction media (Murashige and Skoog, 1962) with varying concentrations of 2, 4-D, namely 1.0, 1.5, 2.0, 2.5, 3.0, and 3.5mg/l. It shows the maximum degree of callus induction at 2 mg/l 2,4-D. The maximum callus induction rate was 86%. Plant regeneration efficacy of in vitro generated plantlets was studied then successfully transferred into soil conditions.