COMPREHENSIVE OVERVIEW OF ADAPTOR PROTEIN RUK/CIN85 ROLES IN CANCER

Aim. This study is focused on a comprehensive overview of mechanisms and processes involved in the acquisition of cancer cell plasticity in a manner dependent on the adapter protein Ruk/CIN85 (in rodents, Ruk — regulator of ubiquitous kinase; in human CIN85 — Cbl-interacting protein of 85 kDa, encoded by SH3KBP1 gene).. Methods. Gene expression was evaluated using RT2-PCR and Western blotting, cell proliferation and survival were analyzed using MTT and/or dye exclusion assays, motility was assessed by scratch test and Transwell assay, enzyme activities were measured using spectrophotometric assays. In vivo metastasis were studies using experimental metastasis model. Conclusion. This study discloses various aspects of cancer cells plasticity, such as EMT, stemness, metabolic changes, ECM components, and drug resistance in dependence on adaptor protein Ruk/CIN85 expression level.