Collagen-Based Gel with Antimicrobial Activity from Leather Waste

The work is focused on obtaining collagen-based gel from leather waste with antimicrobial activity for further use with biomedical application and as dressings for wounds. Two antibiotics - macrolide azithromycin and phenicol chloramphenicol were tested using both laboratory strain and clinical isolates of Staphylococcus species from patients with wounds in Kyiv region. Collagen matrix was obtained from delimed pelt leather waste by acetic acid extraction. The biofilm assay and MTT assay were applied to study the effect of antimicrobial component in collagen-based gel. The reduced growth rate, cells attachment level and cells metabolic activity were the evidences of strains inhibition and biofilm development prevention.