Validation of Method for 4-Chloroaniline and 4,4'-Bi-O-Toluidine from Dyed Leather

Toxicity assessment of leather products designed to obtain leather goods must be in accordance with legislation and environmental standards which require the use of chemicals from semi-finished leather processing. Azo dyes will be checked for two banned aromatic amines (4- chloroaniline and 4,4'-bi-o-toluidine) which can be found as a result of splitting azo dyes used to dye the leather. The method will be validated by gas chromatography (GC-MS) and the following parameters will be tested: accuracy (trueness and reliability), precision, sensitivity, detection limit, limit of quantification, working range, linearity.