Collagen-Indomethacin-Hydroxyapatite Spongious Forms for Bone Reconstruction Treatment

The aim of this study was to prepare and characterize collagen spongious forms for bone tissue engineering. The ideal scaffold has almost the same structural and biological functions as a naturally occurring extracellular matrix in terms of physical structure and chemical composition Having about 30% collagen and 70% hydroxyapatite, to mimic bone composition, the obtained matrices were characterized by water up-take, enzymatic degradation, scanning electron microscopy and indomethacin release from supports. The results showed that the composites based on collagen and hydroxyapatite absorbed less water than the ones with indomethacin. The degradation process showed that indomethacin inhibits collagenase solution, degradation taking place in more than one week. The pore sizes are proper for bone tissue engineering and the release of drug is fast in first 4 hours and slowly next hours. The results showed us that the combination between collagen, indomethacin and hydroxyapatite is a promising spongious form, slowly degraded in vitro, with specific absorbance properties, with proper pore sizes and porosity and which can release the drug rapidly, ensuring a diminution of the inflammation and of the associated pain; this formulation could be successfully used in bone regeneration.