Cajal Bodies in Developing Histaminergic Neurons of the Rat Brain |Biomedgrid

Objective: The aim of the study is to assess the features of Cajal bodies formation in rat brain histaminergic neurons from the 5th to the 45th days of postnatal ontogenesis. Material and methods: The scientific work was performed on the offspring of outbred white rats (12 animals). The decapitation of rats was carried out on the 5th, 20th and 45th days after birth. We used histological, electron microscopic, morphometric, and statistical research methods in the work. The obtained data were processed by nonparametric statistics. Results: From the 5th to the 45th days of the postnatal period, in the nuclei of rat hypothalamus histaminergic neurons, Cajal bodies, mainly in contact with the nucleolus or lying freely, are detected. In the process of establishing close association with the nucleolus, these structures are attached to it, forming a “cap” on its surface. On the 20th day, an increase in the degree of nucleoli association with Cajal bodies is revealed, which is manifested by the formation of perinucleolar ring clusters from Cajal bodies in the form of rosettes. Conclusion: During the described period of postnatal ontogenesis of histaminergic neurons, Cajal bodies do not undergo significant changes in shape and size, which could be related to the age of the animals. The frequency of occurrence of these structures decreases by almost 2.6 times.