Assessment of Possible Link Between Uropathogenic Escherichia Coli and Azoospermia in Male Mice |Biomedgrid

Controversies and debates still prevail in current literature concerning the role of bacterial infection in male infertility. A handful of studies have discovered a correlation between bacteria and azoospermia condition in which the male discharge is utterly devoid of spermatozoa. In line with this, the present study was designed to evaluate the influence of Escherichia coli on spermatogenesis. A single dose (108 cfu/20ul) of uropathogenic Escherichia coli causing sperm agglutination was administered intratesticularly and animals were euthanized on Day 7. Various parameters viz. body weight profile, TSI (%), bacterial load, spermiogram analysis, histopathological changes were evaluated in comparison to the control group administered with PBS. The administration of E. coli led to impairment of spermatogenesis as evident by absence of spermatozoa in right side of testis and revealed by the histopathological examination of the testes showing altered histology. The histology depicted hypospermatogenesis with significant alterations in tissue architecture with no spermatozoa as compared to the control group, indicating azoospermia. If E. coli is injected into the testicles, it can colonise there and eventually lead to azoospermia in the male. This research provides new perspectives and allude to a potential explanation by which uropathogen infection reduces a man's reproductive vitality and causes long-term damage to the male reproductive system.