THE EFFECTS OF HELIUM-NEON LASER WITH DIFFERENT ENERGY DOSES ON CRYOPRESERVED RAM SEMEN QUALITY IN VITRO EXAMINATION

The aim of the study was to investigate the effects of different energy doses of helium?neon (He?Ne) laser irradiation on the functions and functional quality of sheep spermatozoa during in vitro liquid storage. In the study of the quality of stored turkey semen was found to be improved significantly following He-Ne laser irradiation and irradiation with HeNe laser prevented their in vitro liquid storage-dependent damage. It was found also in another study, that irradiation increased the sperm motility index, viability, and cell energy charge. Frozen ram sperm samples in the present study, were thawed in a water bath at 37°C for 30 seconds. Samples pool was divided into three aliquots: one represented the control and the others two was irradiated with He?Ne laser at two different energy dose (3.96 and 6.12 J/cm2 ). Motility, viability, osmotic-resistance, acrosomial and DNA intactness were evaluated. The lower dose of laser energy resulted to be ineffective (P<0.05) than other irradiated samples and control. No significant difference between the control and the irradiated samples for viability and osmotic resistance, acrosome integrity and DNA integrity was found. However, the semen samples irradiated with 6.12 J/cm2 showed a slight increase in sperm progressive motility, viability, osmotic resistance, acrosome and DNA integrity, respect to the semen samples irradiated at low energy doses and control semen samples. Further studies are needed to assess the effect of higher doses of He-Ne laser irradiation on the improvement of the quality ram semen after freezing-thawing process.