Immunohistochemical localization of transient receptor potential vanilloid-type 5 in ameloblasts of Swiss mice

Objective. To determine the localization of transient receptor potential vanilloid 5 (TRPV5) in the ameloblasts of Swiss mice. Materials and methods. In vitro experimental study where paraffin sections of 12 continuously growing incisors of 7-day-old male Swiss mice were analyzed. Developmental stages of dental enamel formation were identified by hematoxylin-eosin staining. Immunodetection of the transient receptor potential vanilloid 5 was performed using the primary polyclonal antibody anti-TRPV5. Observations were carried out using a Leica DM 500 microscope. Results. The different stages involved in the formation of dental enamel were identified, including the secretory and maturation stages. In them, it was evidenced that the ameloblasts were elongated cells with oval nuclei in a proximal position and with developed areas of medial and distal secretion. TRPV5 immunolabeling was visualized in the cell membrane and cytoplasm of the secretory and maturation ameloblasts of all mice tested. TRPV5s were also immunolocalized in odontoblasts, vascular endothelium, and pulp cells. Conclusions. The transient receptor potential vanilloid 5 is located on the secretory and maturing ameloblasts of Swiss mice. In particular, TRPV5s are immunodetected in odontoblasts, vascular endothelium, and dental pulp cells.