HUMAN MESENCHYMAL STEM CELLS: A COMPARITIVE STUDY OF THEIR ISOLATION, EXPANSION AND CHARACTERISATION FROM THE BLOOD AND MATRIX OF THE UMBILICAL CORD
Journal: Stanley Medical Journal (SMJ) (Vol.1, No. 1)Publication Date: 2014-09-10
Authors : Rosy Vennila; R. Secunda; M. Rajasundari; S. Jeswanth; R. Surendran;
Page : 21-24
Keywords : Mesenchymal stem cells; Wharton’s jelly; Explant method; Umbilical cord blood; Density gradient Comparison; mesenchymal; stem cells; wharton's; explant; umbilical;
Abstract
Mesenchymal stem cells (MSCs) are attractive tools for tissue engineering targeting clinical transplantation due to their regeneration potential and immuno-suppressive capacity. MSCs from bone marrow are widely used, but their harvest requires an invasive procedure. The umbilical cord which is discarded at birth, is a source for both cord blood as well as matrix (also known as Whartonâ?™s jelly), which can be used to derive mesenchymal stem cells. We used both Umbilical cord blood (UCB) and Whartonâ?™s Jelly (WJ ) to isolate MSCs by Density gradient separation and Explant culture method respectively. The same induction system (DMEM low glucose with 20% FBS) was used and the efficiency of isolation, proliferation and expansion were compared. The Whartonâ?™s jelly derived MSCâ?™s (WJMSCs) showed a relatively rapid proliferation and hence is a better source when compared to cord blood. The method of isolation of MSCs from Whartonâ?™s jelly is non controversial, involves a painless procedure (compared to bone marrow aspiration, which is conventionally used) and relatively easier to process than cord blood. Hence Whartonâ?™s jelly is a good source for MSCs with potential therapeutic applications in the future. Mesenchymal stem cells,Wharton’s jelly,Explant method,Umbilical cord blood,Density gradient Comparison,mesenchymal,stem cells,wharton's,explant,umbilical
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