Porcine Placenta Peptide Increases the Proliferation and Migration of Human Gingival Epithelial Cells and the Secretion of Epidermal Growth Factor |Biomedgrid

Placenta tissue contains multiple biologically active substances, such as amino acids, trace elements, hormones, and cytokines required for fetal growth. The role of the placenta as a health improvement product can also be as placenta extracts containing different peptides. The beneficial use of placenta membrane has been described with periodontal tissue regeneration. These positive effects could be due to the presence of biological mediators in the placenta membrane and peptides. We explored the effect of a specific placenta extract (molecular weight cutoff of 30kDa) housing five small peptides, LSSPATLNSR, ASISLPR, ILLEVNNR, ESLITLIEK, and QPLLLDDR, on human gingival epithelial cell behavior. Results show that placenta peptide at 0.1-100μg/mL significantly increased the proliferation of epithelial cells at 48 and 96h, and at 10 and 100μg/mL accelerated cell migration, contributing to faster wound closure than that in the control. Cell proliferation and migration were supported by increased Epidermal Growth Factor (EGF) secretion and a decrease in metalloproteinase MMP-2. This is the first study to report a novel biological activity of placenta peptide as a wound-healing contributor in human gingival epidermal cells.