CONFIRMATORY METHOD OF RESORCYLIC ACID LACTONES IN URINE AND TISSUE BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY

The spread of antibiotic resistances and the appearance of multiple-antibiotic-resistant pathogenic bacteria has been recognized by the WHO as a serious problem that complicates medical treatment of bacterial infections. In last years, when campylobacteriosis became the most frequently disease the antibioresistance of this microorganism represents a serious problem. Campylobacter’s antibioresistance was carried out through the determination of minimal inhibitory concentration using Sensititre system. This analysis were performed in panels dedicated for these microorganisms. There were tested 132 Campylobacter jejuni and Campylobacter coli strains previously isolated from chicken meat. The species of Campylobacter was perform according ISO 10272/2006.Of the 132 analyzed Campylobacter strains, 39 strains were susceptible to all antimicrobial substances tested, and 93 strains showed resistance to at least one antimicrobial agent; 4 strains were resistant to 6 antimicrobial substances. C. coli showed a higher degree of resistance than C. jejuni to all antimicrobial substances that were tested.