HISTOLOGY OF NEW ZEALAND MALE RABBIT LIVER AFTER TREATMENT WITH SEVERAL TYPES OF BELUNTAS LEAF TANNIN EXTRACT

This study examined New zealand rabbits organ damage and liver histology after administering beluntas leaf tannin extract. The type of research is experimental research. The research was conducted at the Chemical Engineering Laboratory of the Malang State Polytechnic, the KesimmaMedica Laboratory of Malang, the Integrated Laboratory, and the Biology Laboratory of the University of Muhammadiyah Malang. The research design used a Completely Randomized Design (CRD) with four treatments and four replications. Treatment of hydrolyzed, condensed beluntas leaf tannin extract, pure tannin, and the control group, namely distilled water, was given by oral probe at a dose of 3 ml/KGB. Histology preparations were made using the paraffin method and Hemotoxylin-Eosin(HE) staining. Liver damage scoring was observed in 3 fields around the central vein; each preparation was observed in 3 fields of view, each of which was killed by 100 hepatocyte cells. Data analysis used average analysis, one-way ANOVA, and Duncans further test. The results showed that treating several tannins affected the damage to New zealand rabbit hepatocyte cells. The results of Duncans analysis showed that the treatment of pure tannins was not significantly different from hydrolyzed tannins and substantially different from the control group and condensed tannins; the control group was not significantly different from condensed tannins. This study found that pure tannins and hydrolyzed tannins had the highest effect on hepatocyte cell damage; condensed tannins were recommended for further research. Suggestions for further research are the potential of steroid tannin derivative compounds, namely single stigmasterol compounds from beluntas leaves, which can be antifertility in vivo, quality, and safety studies.