Hoficin and remaxol effects on metabolic intoxication and lipid peroxidation in patients with chronic non-calculous cholecystitis on the background of diffuse liver diseases

Relevance. The effect of the combined use of hoficin and remaxol on the indicators of metabolic intoxication and lipid peroxidation was studied in patients with chronic non-calculous cholecystitis on the background of diffuse liver diseases. Materials and Methods. The effect of the combined use of hoficin and remaxol was studied in 63 patients diagnosed with chronic non-calculous cholecystitison the background of acute chronic diffuse liver disease (CDLD), aged 20 to 53 years. Patients, by random distribution by age, gender, and severity of the clinical course of chronic non-calculous cholecystitis against the background of сhronic diffuse liver damage, were divided into two equivalent groups–the analyzed group, according to which the combined use of hoficin and remaxol and the control group using conventional treatment, after which the results were compared. In patients in the analyzed group, the level of middle molecules (MM) and the concentration of lipid peroxidation (POL) products, final malonic dialdehyde (MDA) and intermediate diene conjugates (DC) in the blood serum were studied. The screening method of V.V. Nikolaichyk was used in the modification of Gabrilovich. To study the level of medium molecules, blood serum was obtained by centrifugation at 4000g for 15 minutes. The method is based on plasma purification from high-molecular peptides and proteins using trichloroacetic acid, a 10% solution, and then the level of medium-­molecular peptides in terms of absorption in a monochromatic light stream was determined using direct spectrophotometry (at a wavelength of 254 nm) of the liquid. Results and Discussion. The content of MDA in blood plasma was estimated by M. Ushiama et al. in reaction with thiobarbituric acid. The studies were carried out in dynamics–before and after treatment. In patients with chronic non-calculous cholecystitis, an increase in serum MM levels and an increase in the concentration of POL products is observed. The use of the herbal preparation hoficin in combination with remaxol contributes to the normalization of clinical and laboratory signs of the disease, normalization of POL products. Under the influence of hoficin in combination with remaxol, the metabolic intoxication syndrome is eliminated, which is manifested by a significant decrease in the level of medium-­weight molecules to the upper limit of the norm, that is, this indicator is completely normalized, and the positive effect of treatment is observed in the first group of patients, unlike patients of the second group, in addition, the relative metabolic constancy of the internal environment of the body is restored. Conclusion. Patients with chronic non-calculous cholecystitis against the background of diffuse liver diseases exhibit endogenous metabolic intoxication syndrome. Increased concentration of LPO products-­MDA and DC indicates activation of biomembrane lipid peroxidation. Including the herbal preparation Hoffitsin in combination with Remaxol in the general treatment course of these patients contributes to normalization of clinical and laboratory disease indicators, medium molecule levels decrease to normal, and LPO products-­MDA and DC normalize. Under the influence of Hoffitsin in combination with Remaxol, metabolic intoxication syndrome is eliminated and relative metabolic constancy of the body’s internal environment is restored.