Comparative Evaluation of Aqueous Microwave and Organic Solvent Extracts of Pulicaria Jaubertii

This study evaluates and compares the bioactive potential of Pulicaria jaubertii extracts obtained via microwave assisted extraction MAE using two aqueous solvents distilled water DW and alkaline deionized water ADW against conventional organic solvent extracts methanol, petroleum ether . The extracts were assessed for yield, phytochemical content, antioxidant capacity, anti inflammatory effects, and anticancer activity. DW MAE yielded the highest extraction efficiency 23.2 , total phenolic content 14.6 mg GAE g , and flavonoid content 9.2 mg QE g . It also exhibited the strongest antioxidant activity IC50 = 38.2 µg mL , maximal inhibition of protein denaturation 28.6 , and red blood cell hemolysis protection 96.15 . Cytotoxicity assays against HepG2 and MCF 7 cell lines confirmed greater potency of aqueous–microwave extracts compared to organic solvent counterparts. GC–MS analysis revealed higher levels of neophytadiene, hexadecenoic acid methyl ester, and oxygenated diterpenes in the DW extract. Findings demonstrate that dual aqueous MAE protocols offer a safe, eco friendly, and effective alternative for producing anti inflammatory and anticancer phytoconstituents from P. jaubertii. Entesar Saleh Shaif Moqbel | Dr. Ravi Pradhan "Comparative Evaluation of Aqueous-Microwave and Organic Solvent Extracts of Pulicaria Jaubertii" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-9 | Issue-5 , October 2025, URL: https://www.ijtsrd.com/papers/ijtsrd97365.pdf Paper URL: https://www.ijtsrd.com/biological-science/zoology/97365/comparative-evaluation-of-aqueousmicrowave-and-organic-solvent-extracts-of-pulicaria-jaubertii/entesar-saleh-shaif-moqbel